Figure 2
From: Osteoclast-independent bone resorption by fibroblast-like cells
Characterization of cultured human prosthesis loosening fibroblasts (PLFs) by flow cytometry. (a) When compared with isotype control staining, analysis for the macrophage lineage marker CD68 showed no surface expression (<0.1%). (b) Also, no expression of the leucocyte common antigen CD45 was found on the cells (<0.1%). (c) However, fluorescent-activated cell sorter analysis with the fibroblast marker D7-Fib revealed the fibroblast nature of the cells (>99%). (d) In addition, more than 99% of the PLFs stained positive for the fibroblast marker AS02.