Figure 3
From: DEK binding to class II MHC Y-box sequences is gene- and allele-specific
Namalwa cell nuclear protein(s) bind to DQA1 and DRA Y-box elements. 5 μg of Namalwa cell nuclear extract (lanes 2–10) and unlabeled oligonucleotide competitors were added to the binding reaction before addition of the radiolabeled DQA1*0101 probe (a) or the radiolabeled DRA probe (b) to define the sequence specificity of nuclear protein binding. In both (a) and (b), lane 1 contains no protein. See Figure 1 for site mutant and CCAAT mutant sequences. pets = peri-ets.