Figure 3
From: Regulation and localization of endogenous human tristetraprolin
Changes in tristetraprolin (TTP) expression by leukocytes after lipopolysaccharide (LPS) stimulation in vitro. (a) LPS induced TTP expression in all leukocytes. Results are expressed as the LPS-induced increase in TTP specific staining over baseline expression. Significant increases are shown with respect to time 0 (*P < 0.05 monocytes or lymphocytes; #P < 0.05 neutrophils; n = 8). Monocyte recovery was poor at time points beyond 4 hours and these results are excluded. (b) Confocal microscopy shows the upregulation of TTP in neutrophils 2 hours after LPS stimulation. Neutrophils before (i-iii) and after (iv-vi) LPS stimulation. (i,iv) transmitted light (differential interference contrast) image of the cell; (ii,v) TTP (FITC); (iii,vi) composite image, nuclear material stained red with propidium iodide.