Regulation and localization of endogenous human tristetraprolin

Table 1 Constitutive expression of tristetraprolin in peripheral blood leukocytes

Cell type	MESF (in thousands) Baseline expression^a	Range
Lymphocyte	9.5 (1.2)	4.45–14.17
Monocyte	35.4 (3.3)*	13.90–44.32
Neutrophil	13.8 (3.3)	9.14–18.28

Intracellular TTP levels were detected using the TTP antibody in conjunction with a fluorescein-labelled antirabbit Ig. Background levels were subtracted from these results and CD14-staining plus forward and side scatter gating permitted specific analysis of TTP expression in the leukocyte populations. Monocytes have a significantly higher constitutive TTP expression than other leukocytes. ^aMean (SE); n = 8. *P < 0.001. MESF = milliequivalents of soluble fluorescein.

ISSN: 1478-6362