Figure 2From: Higher susceptibility to Fas ligand induced apoptosis and altered modulation of cell death by tumor necrosis factor-α in periarticular tenocytes from patients with knee joint osteoarthritisCharacterization of osteoarthritis (OA) tenocytes by flow cytometry. (a) When compared with isotype control staining, analysis for the macrophage lineage marker CD68 showed no surface expression (<1%). (b) Also, no expression of the leucocyte common antigen CD45 was found on the cells (<0.1%). (c) However, fluorescence activated cell sorting analysis with the fibroblast marker AS02 showed positive staining of all the cells (>99%). (d) In addition, more than 99% of the tenocytes stained positive for the fibroblast markers D7-Fib. (e) Intracellular staining for prolyl-4-hydroxylase (>99%) confirmed the fibroblast-like nature of the tenocytes.Back to article page