Method
|
Higher in naïve DA rats
|
Lower in naïve DA rats
|
Higher in pristane-treated DA rats
|
Lower in pristane-treated DA rats
|
---|
Affymetrix (pooled samples)
|
15 (8800)
|
24 (8800)
|
38 (8800)
|
65 (8800)
|
Custom-made chips
|
19 (170)
|
2 (170)
|
2 (170)
|
23 (170)
|
Real-time PCR
|
0
|
0
|
0 (5)
|
3 (5)
|
FACS
|
4 (7)
|
3 (7)
|
3 (7)
|
2 (7)
|
ELISA
|
Total IgE and IgM (3)
|
0 (3)
|
Total IgM (3)
|
Total IgG (3)
|
- To be regarded as differentially expressed with statistical significance, a gene had to fulfil the following threshold values: Affymetrix (pooled samples), differentially expressed with a fold change > 3.0; custom-made oligomer glass chips, differentially expressed with one group t-test P < 0.05; real time PCR, differential expression with Mann–Whitney P < 0.05; FACS, differential geometric mean values with a Mann–Whitney P < 0.05; ELISA, differential plasma concentrations with a Mann–Whitney P < 0.05. The values within parenthesis represent the total number of genes analyzed using the method. ELISA, enzyme linked immunosorbent assay; FACS, fluorescence activated cell sorting.