Figure 1

CCR9 and CCL25 expression in the RA synovial tissues. (A) Western blots of CCR9 protein expression in three RA and three OA synovial tissues. Ratio of CCR9/β-actin protein level is indicated below. (B-J) Sections of RA synovial tissue were double-stained with CCR9 and CD68, cadherin-11 and DC-LAMP (B, CD68; C, CCR9; D, merged B with C; E, cadherin-11; F, CCR9; G, merged E with F; H, DC-LAMP; I, CCR9; J, merged H with I). Bar, 20 μm. (K) Western blots of CCL25 protein expression in four RA and four OA synovial tissues. Ratio of CCL25/β-actin protein level is indicated below. (L-N) Sections of RA synovial tissue were double-stained with CCL25 and CD68 (L, CD68; M, CCL25; N, merged L with M). Bars, 20 μm. CCL, chemokine (C-C motif) ligand; CCR, CC chemokine receptor; DC-LAMP, dendritic cell lysosome-associated membrane glycoprotein; OA, osteoarthritis; RA, rheumatoid arthritis.