Figure 4From: Subchondral bone influences chondrogenic differentiation and collagen production of human bone marrow-derived mesenchymal stem cells and articular chondrocytesImmunofluorescence staining of collagens. Representative immunofluorescence staining of collagens I (A), II (B), III (C) and X (D) and of Alcian blue (E) in fibrin gels of BMSC, mixed cultures (BMSC + chondrocytes 1:1) and chondrocytes after 28 days of co- and triculture with OAB (FB) or monoculture (F) (Scale bar is 100 μm). BMSC, bone marrow-derived mesenchymal stem cells; F, fibrin gel-embedded monocultures (without subchondral bone explants); FB, fibrin gel-embedded co- and tricultures together with subchondral bone explants; OAB, osteoarthritic subchondral bone.Back to article page