Immunostaining of biglycan, 4',6-diamidino-2-phenylindole (DAPI) and human nuclei (HuNu) in the intervertebral disc (IVD). Immunostaining of HuNu (A-C), biglycan (D-F), DAPI (G-I) and their colocalization (J-L) in IVDs are shown. The images are representative of placebo (A,D,G,J) and adipose mesenchymal stem cell (ADSC) mice (B,E,H,K) at 12 weeks from injection, and of wild type (WT) (C,F,I,L) animals at similar age. In the inserts of panel E and K, a magnification of biglycan immunostainings are shown. Scale bar, 50 μm.