Engraftment and co-localization of human nuclei (HuNu)-positive cells with biglycan. (A) Representative images of HuNu immunostaining of a disc adjacent to the injected one (vertebrae L1 to L2) in placebo (upper panel) and adipose mesenchymal stem cell (ADSC)-implanted (lower panel) group are shown. Black arrows indicate the disc region were HuNu-positive cells are present. (B) The number of HuNu- (light gray), biglycan- (black) and aggrecan- (dark grey) positive cells in intervertebral discs from ADSC-transplanted and placebo groups are shown. Positive cells were measured in the whole discs of five individual animals transplanted with ADSCs (1 to 5), or injected with saline (6 to 10) at 12 weeks of treatment. Positive cells in five wild type (WT) animals of similar age are shown on the right. Data are the mean ± S.D.