Figure 2

Effect of lysophosphatidic acid receptor 1 on pseudoemperipolesis and migration of rheumatoid arthritis fibroblast-like synoviocytes. After preincubation of cocultured rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs) and CD4⁺ T cells (A) and (B) or CD8⁺ T cells (C) and (D) or CD19⁺ B cells (E) and (F) with a lysophosphatidic acid (LPA) receptor 1 inhibitor (LA-01; 0, 1 or 10 nM) for 30 minutes, the cells were stimulated with 10 ?M LPA for 12 hours. Control: no stimulation with LPA. After the cells were washed, the number of lymphocytes beneath FLSs was counted. Representative photomicrographs of three independent experiments are shown (A, C and E). Arrows indicate the lymphocytes beneath FLSs. Original magnification, ×200. Data on the number of lymphocytes beneath FLSs are presented as one of three independent experiments analyzed in triplicate (B, D, and F). Data are presented as the mean ± SEM. *P < 0.05 vs control or LA-01 0 nM (B, D, F).