Rheumatoid synovial fibroblasts degrade centrally and at focal adhesions. Rheumatoid synovial fibroblasts were allowed to adhere for 2 hours to coverslips coated with fluorescently labeled gelatin. Coverslips were fixed and stained for cortactin, vinculin and paxillin. Images are representative of gelatin degradation and staining for cortactin (A), vinculin (B) and paxillin (C) in three independent experiments in which we used rheumatoid synovial fibroblasts from two different patients. Images were made at 400× magnification, and the bar represents 50 μm. To demonstrate degradation at focal adhesions, boxes indicate examples of areas where gelatin degradation did not colocalize with cortactin (A), a marker of invadopodia, as well as where gelatin degradation did colocalize with vinculin (B) and paxillin (C), two markers of focal adhesions.