Figure 3From: Focal adhesion kinase is required for synovial fibroblast invasion, but not murine inflammatory arthritisFocal adhesion kinase inhibitors do not alter focal matrix degradation, but do reduce migration of rheumatoid synovial fibroblasts. (A) Rheumatoid synovial fibroblasts were treated with PF-562,271, PF-573,228 or dimethyl sulfoxide (DMSO) as the vehicle control, plated on fluorescently labeled gelatin, fixed after 5 hours and stained for vinculin. Representative images of matrix-degrading cells from each condition are displayed (n = 3 independent experiments using cell lines from two different patients). Images were created at 400× magnification, and the bar represents 50 μm. (B) Left panel depicts a scoring system for gelatin degradation: 1 = no peripheral degradation (note: for quantitative analyses in (C) and (D), only cells that were completely within the field of view were scored), 2 = central and severe peripheral degradation, 3 = severe peripheral degradation and no central degradation and 4 = mild peripheral degradation and no central degradation. Cells treated as in (A) were scored for degradation as described in for (B). (C) and (D) Graphs show the average scores ± SEM for central (C) and peripheral (D) degradation (n = 3 independent experiments using cell lines from two different patients). (E) Rheumatoid synovial fibroblasts were treated with PF-562,271, PF-573,228 or vehicle control (DMSO) and allowed to migrate for 5 hours across a transwell. The graph shows average number ± SEM of migrated cells per microscopic field at 100× magnification (n = 4 independent experiments using cell lines from three different patients, *P < 0.05 by one-way analysis of variance).Back to article page