Table 2 Primers for quantitative real-time PCR
Name | Sense primer (5'-3') | Antisense primer (5'-3') |
|---|---|---|
β-actin | GGACTTCGAGCAAGAGATGG | TGTGTTGGCGTACAGGTCTTTG |
IL-6 | CTGCGCAGCTTTAAGGAGTTC | CAATCTGAGGTGCCCATGCTA |
IL-8 | GTGCAGAGGGTTGTGGAGAAGTTT | TCACTGGCATCTTCACTGATTCTTG |
IL-1β | CCAGCTACGAATCTCCGACC | CATGGCCACAACAACTGACG |
TNF-α | GCTAAGAGGGAGAGAAGCAACTACA | GAAGAGGCTGAGGAACAAGCA |
MMP-3 | TTTCCAGGGATTGACTCAAAGA | AAGTGCCCATATTGTGCCTTC |
MMP-13 | TCCTGGGCCAAATTATGGAG | TTGCCGGTGTAGGTGTAGATAGGAA |
RANKL | ACCAGCATCAAAATCCCAAG | CCCCAAAGTATGTTGCATCC |
NF-κB | ATGTGGAGATCATTGAGCAGC | CCTGGTCCTGTGTAGCCATT |
ERK | CTACACGCAGTTGCAGTACAT | CAGCAGGATCTGGATCTCCC |
p38 | CCCGAGCGTTACCAGAACC | TCGCATGAATGATGGACTGAAAT |