Figure 1

Histological evaluation of osteoarthritis (OA). (A, B) Representative images of toluidine blue staining from dimethyl sulphoxide (DMSO) (A) and rapamycin (B) treated mice at 8 and 12 weeks after destabilization of the medial meniscus (DMM) or sham surgery. Boxed areas in the toluidine blue stained image at the left side (x4) indicate the regions shown in the enlarged toluidine blue stained area at the right side (x20). Scale bar = 500 μm (x4) and 100 μm (x10). Arrows indicate the osteoarthritic change after DMM surgery. (C) Graph indicating the summed OA scores. Summed OA scores were calculated from all four quadrants and eight sections from each knee. ***P <0.001, **P <0.01.