Figure 3

The effect of rapamycin on vascular endothelial growth factor (VEGF) expression in the articular cartilage. (A) Representative images of immunostaining for VEGF (green). Scale bar = 20 μm. (B) Quantification of VEGF-positive cells in the cartilage was calculated. (C) Graph indicating quantitative RT-PCR for VEGF in dimethyl sulphoxide (DMSO)- and rapamycin-treated mice at 8 and 12 weeks after destabilization of the medial meniscus (DMM) or sham surgery. *P <0.05, **P <0.01, ***P <0.001.