Figure 6

IL-1β, SB203580 and SP600125 modulated UDP-glucose dehydrogenase ( UGDH ) gene expression. Human articular chondrocytes were pretreated with SB203580 (20 μM) and SP600125 (10 μM) for 30 minutes and/or subsequently treated with IL-1β (10 ng/mL) for another 48 h. Total GAG (A), the mRNA level of UGDH, specificity protein 1 (Sp1), Sp3 and Krueppel-related zinc finger protein c-Krox (c-Krox) (B), and the protein level of UGDH and Sp1 (C, D) was also detected. Values are presented as mean ± standard error of the mean from at least three independent experiments. *P <0.05, **P <0.01 versus control, ^#P <0.05, ^##P <0.01 versus the IL-1β group.