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Table 2 The primers used in the study

From: UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis

Target genes Gene ID Sequence Annealing (°C) Product size (bp)
UGDH 2582 F: 5′-CAGGCTATGTTGGAGGACCC-3′ 60 162
R: 5′-TCGACAGGATTCTACCACTTCTT-3′
Sp1 6667 F: 5′-ATGGACAGGTCAGTTGGCAG-3′ 60 89
R: 5′-CTGCATTGGGGCTAAGGTGA-3′
Sp3 6670 F: 5′-CAGTCAGCAGATGGTCAGCA-3′ 60 185
R: 5′-CCCTGAACCTGGACTTGACC-3′
c-Krox 51043 F: 5′-CGGTGTTCGATTCACCAGGA-3′ 60 134
R:5′-GCAGGTGCATGTGGTTCTTG-3′
GAPDH 2597 F: 5′-GAAATCCCATCACCATCTTCCAG-3′ 60 313
R:5′-GAGTCCTTCCACGATACCAAAG-3′
  1. UGDH, UDP-glucose dehydrogenase; Sp1, specificity protein 1; Sp3, specificity protein 3; c-Krox, krueppel-related zinc finger protein cKrox; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.