Table 2 The primers used in the study
Target genes | Gene ID | Sequence | Annealing (°C) | Product size (bp) |
|---|---|---|---|---|
UGDH | 2582 | F: 5′-CAGGCTATGTTGGAGGACCC-3′ | 60 | 162 |
R: 5′-TCGACAGGATTCTACCACTTCTT-3′ | ||||
Sp1 | 6667 | F: 5′-ATGGACAGGTCAGTTGGCAG-3′ | 60 | 89 |
R: 5′-CTGCATTGGGGCTAAGGTGA-3′ | ||||
Sp3 | 6670 | F: 5′-CAGTCAGCAGATGGTCAGCA-3′ | 60 | 185 |
R: 5′-CCCTGAACCTGGACTTGACC-3′ | ||||
c-Krox | 51043 | F: 5′-CGGTGTTCGATTCACCAGGA-3′ | 60 | 134 |
R:5′-GCAGGTGCATGTGGTTCTTG-3′ | ||||
GAPDH | 2597 | F: 5′-GAAATCCCATCACCATCTTCCAG-3′ | 60 | 313 |
R:5′-GAGTCCTTCCACGATACCAAAG-3′ |