Western blot (WB) analysis of GCA patients’ and controls’ TASMC lysates. WB analysis of GCA patients’ (n = 6) and controls’ (n = 4) TASMC lysates for NTs and NT receptors (A) and for Akt, phospho-Akt, ERK 1/2 and phospho-ERK 1/2 and histograms for phospho-Akt /Akt/ actin ratio (B). Panels are representative examples of WB analysis. (C) Transcription of NTs and NT receptors by TASMCs from GCA patients (n = 6) and controls (n = 6) cultured with 10% FCS. The neuroblastoma cell line SH-SY-5Y (S) was used as a positive control. Constitutively expressed GAPDH is a control of PCR efficiency. Panels are representative examples of the transcription assay. NC: negative control. (D) qRT-PCR analysis of NT and NT receptor expression in TASMC cultures from GCA patients (n = 6) and controls (n = 6), normalized to HPRT gene expression. Akt, protein kinase B; ERK, extracellular signal-regulated kinase FCS, foetal calf serum; GCA, giant-cell arteritis; NGF, nerve growth factor; NT, neurotrophin; PCR, polymerase chain reaction; TASMC, temporal artery VSMC; VSMC, vascular smooth muscle cell.