Sera and supernatant concentrations of NGF and BDNF from GCA patients and controls. (A) NGF and BDNF sera concentrations from patients with GCA (n = 30) compared to controls (n = 48) with a sera dilution of 1:100. (B) NGF TASMC-related supernatant concentration from GCA patients (n = 8) compared to controls (n = 8). Supernatants were collected when VSMC reached 80% confluence, between passages 3 and 5. Experiments were performed in duplicate, with three independent ELISA assays and data are presented as pg/mL. BDNF, brain-derived neurotrophic factor; ELISA, enzyme-linked immunosorbent assay; GCA, giant cell arteritis; NGF, nerve growth factor; TASMC, temporal artery VSMC; VSMC, vascular smooth muscle cell.