Figure 6

Gene expression analyses in synovial cells after overexpression of miR-210. (A) Real-time PCR analysis of collagen type 1 alpha 1 (Col1a1), collagen type 2 alpha 1 (Col2a1), vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF2) at 7 days after in vitro transfection of synovial cells. Expression of Col2a1 was not detected in both groups. Expression of VEGF and FGF2 was significantly higher than in the control group (*P < 0.05, N.S. (no significant difference), n = 6 for each group). (B) Immunohistochemical analysis indicates that VEGF and FGF2 were highly expressed in the miR-210 group (bar = 100 μm). These results demonstrated that miR-210 could enhance the expression of VEGF and FGF2 expression in synovial cells. ds, double stranded.