A comparison of two popular sequence enrichment methods. (A) For amplicon enrichment, PCR primers specific to the region of interest are used to amplify the target area. (B) These PCR products are then prepared for sequencing via ligation with sequencer-specific DNA molecules (adapters). (C) Molecules are then ready for sequencing. (D) For hybridization enrichment, the entire genome is sheared into small fragments which are subsequently ligated to sequencer-specific adapter DNA molecules. (E) Biotinylated oligomers that have been designed to be complementary to the region of interest are incubated with the previously generated sequencing library. (F) Captured molecules from the region of interest are pulled down using streptavidin-coated magnetic beads. DNA molecules are then eluted and ready for sequencing (C).