Frequency of circulating follicular helper T cell (cTfh) subsets in patients with rheumatoid arthritis (RA). (A) Frequency of circulating Tfh-Th1, Tfh-Th2 and Tfh-Th17 cells in R-a, RA-r and HC. Representative dot plots demonstrate CXCR3 and CCR6 expression in cells gated for CD3, CD4 and CXCR5. (B). Overabundance of Tfh-Th2 and Tfh-Th17 cells over Tfh-Th1 cells in RA-a and RA-r patients. (C) Relation of cTfh subset proportions or ratio with disease activity as determined by disease activity in 28 joints (DAS28) score . *P <0.01 versus HC. Tfh, Follicular helper T cells; Tfh-Th1 cells, CXCR5 + CXCR3 + CCR6- T cells; Tfh-Th17 cells, CXCR5+ CXCR3-CCR6+ T cells; Tfh-Th2 cells, CXCR5 + CXCR3-CCR6- T cells.