Figure 4

Activation of NF-κB pathway evaluated by Western blot analysis and NF-κB DNA binding assay. (A-D) Synoviocytes obtained from patients with developmental dysplasia of the hip (DDH) were incubated with 100 nM substance P (SP) or 1 nM calcitonin gene-related peptide (CGRP) within 1 hour. Nuclei cell proteins were subjected to Western blot analysis (A) and (C) with antibodies against phosphorylated p65 (p-P65). (B) and (D) Densitometric analysis of Western blotting data derived from (A) and (C). *P < 0.05 versus control. (E) and (F) Nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) p65 and DNA binding assays. Synoviocytes were pretreated with L-703606 or MK-3207 for 2 hours, followed by 1 hour of stimulation with SP (100 nM) or CGRP (1 nM). *P < 0.05 versus control. #P < 0.05 versus synoviocytes stimulated with SP or CGRP alone.