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Table 2 Sequences of PCR primers related to neuropeptides and inflammatory cytokines included in this study a

From: Increasing expression of substance P and calcitonin gene-related peptide in synovial tissue and fluid contribute to the progress of arthritis in developmental dysplasia of the hip

Gene

Forward primer (5′-3′)

Reverse primer (5′-3′)

TAC1 (SP)

GGTACGACAGCGACCAGATCA

CCCGTTTGCCCATTAATCCA

CGRP

GCAAGAGAGAGAGGGCTCCA

TTTCTTTCCAGGTGCTCCAA

IL-1β

CCTTGTCCTGCGTGTTGAAAGA

AAACTCAGACGGGTCAAGGG

IL-6

GATGAGTACAAAAGTCCTGATC

CTGCAGCCACTGGTTCTGT

TNF-α

CAGCCTCTTCTCCTTCCTGAT

GCCAGAGGGCTGATTAGAGA

MMP-13

CCCTTGATGCCATTACCAGTC

TCCGCATCAACCTGCTGAG

MMP-3

TGATGAACAATGGACAAAGGATAC

CTGTGAGTGAGTGATAGAGTGG

TIMP-1

TGCCGCATCGCCGAGAT

ATGGTGGGTTCTCTGGTG

β-actin

CATCAAGAAGGTGGTGAAGCAG

TGTAGCCAAATTCGTTGTCATACC

  1. aCGRP, Calcitonin gene-related peptide; IL, Interleukin; MMP, Matrix metalloproteinase; TIMP, Tissue inhibitor of metalloproteinase; TNF, Tumor necrosis factor.
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Arthritis Research & Therapy

ISSN: 1478-6362

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