Figure 5
rAAV2 mediated cysC gene transfer into rabbit joints. rAAV vectors were administered 4 weeks post OA induction and the synovium was collected 7 weeks after vector injection. (A) Quantitation of rAAV vector genomes (VGs) and mCysC mRNA levels in the synovium. Detection of BGH polyA sequence by RT-PCR assay was expressed as copies per 1 x 106 cells. (B) Analysis of rabbit catK and cysC mRNA levels in the synovium 11 weeks post ACLT. Values in treatment groups were expressed as fold increase over normal (sham/PBS). All values represent the group mean ± standard deviation (SD) (n = 7/group). Significance by two-tailed Student’s t test. ACLT, anterior cruciate ligament transection; BGH, bovine growth hormone; (m)CysC, (mouse) cystatin C; OA, osteoarthritis; PBS, phosphate-buffered saline; rAAV, recombinant adeno-associated virus.