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Figure 6 | Arthritis Research & Therapy

Figure 6

From: Overexpression of cystatin C in synovium does not reduce synovitis or cartilage degradation in established osteoarthritis

Figure 6

Effect of cysC transfer on synovial cathepsin activity. (A) Cathepsin activity in rabbit synovium at 11 weeks. Activity by RFU values from each treatment group was normalized by total protein content and represent the group mean ± standard deviation (SD) (n = 7/group). Significance by two-tailed Student’s t test. (B) Characterization of cathepsin activity in the rabbit synovium. ACLT/PBS lysates (n = 3 rabbits) were analyzed for activity in the presence of 0.5 and 0.1 μM (triangle) concentration of E-64, mCysC or CA-074 (catB-specific inhibitor). Purified catK and catB (50 nM each) proteins were tested for inhibition by CA-074 as controls. Data are shown as percentage of activity compared to no inhibitor. ACLT, anterior cruciate ligament transection; E-64, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane; (m)CysC, (mouse) cystatin C; PBS, phosphate-buffered saline; RFU, relative fluorescence units.

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