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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Deficiency of fibroblast activation protein alpha ameliorates cartilage destruction in inflammatory destructive arthritis

Figure 3

Functional role of fibroblast activation protein (FAP) on synovial fibroblast behavior. (A) Micophotographs of in vitro migration assays from human tumor necrosis factor alpha transgene (hTNFtg) and FAP knockout (fap−/−) hTNFtg SF demonstrating gap closure after 56 hours. (B) Quantification comparison of gap closure between wild-type (wt) and fap−/− as well as hTNFtg and fap−/− hTNFtg SF at indicated time points. All data are means ± standard error of the mean (SEM) of three mice each. (C) Representative pictures of in vitro attachment of hTNFtg and fap−/− hTNFtg SF on wt cartilage, pretreated with 1 ng/ml interleukin 1 beta (IL-1β), after 14 hours. Cartilage and attached cells were stained with hematoxylin. (D) Quantitative assessment of attached synovial fibroblasts (SF) from hTNFtg and fap−/− hTNFtg mice on both non-treated (white bars) and treated (black bars) wt cartilage. All data are means ± SEM of three mice each (* P <0.05).

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