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Figure 5 | Arthritis Research & Therapy

Figure 5

From: CCAAT/enhancer-binding protein β promotes receptor activator of nuclear factor-kappa-B ligand (RANKL) expression and osteoclast formation in the synovium in rheumatoid arthritis

Figure 5

ATF4 is constitutively expressed in rheumatoid arthritis (RA) synovium, but translocated into the nucleus by C/EBPβ-LIP. (A) Expression and distribution of ATF4 in RA synovium by immunofluorescence staining. Original magnification, 200×. (B) Expression of ATF4 protein levels from fibroblast-like synoviocytes from the synovium of RA patients (RA-FLS) by western blotting. ATF4 protein in HeLa cells was used as a positive control. (C) Expression of ATF4 mRNA of RA-FLS overexpressed C/EBPβ by quantitative RT-PCR. Isolated RA-FLS were transfected with adenovirus expression vectors for C/EBPβ-LAP, −LIP or LacZ control. The level of ATF4 mRNA relative to LacZ control is shown. (D) Synovial tissue from RA patients was cultured in serum-free medium for 24 hours and then replaced with fresh medium containing adenovirus expression vectors for C/EBPβ-LIP or LacZ control for a further 72 hours. Immunostaining for ATF4 was performed. Original magnification, 400 × .

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Arthritis Research & Therapy

ISSN: 1478-6362

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