-knockout mice show increased bone mineral density and bone volume in subchondral and trabecular bone. (A) Micro-CT three-dimensional coronal reconstructions of mouse knees after a sham operation (left knees) or destabilization of the medial meniscus (DMM) joint (medial femorotibial) showing subchondral bone changes. (B) Bone volume/tissue volume ratio (BV/TV, %) measurement in the subchondral bone of knees of wild-type (WT) and SOST-knockout (SOST-KO) mice (n = 5 animals at each time point). (C) Bone mineral density (BMD) measurement in the whole knees of WT and SOST-KO mice (n = 10 animals at week 0 and n = 5 independent animals at weeks 4 and 6 for each genotype). (D) Micro-CT three-dimensional coronal reconstructions of sham knees and knees with DMM showing osteophytes (red circle). (E) Tissue volume measurement of newly formed osteophytes from the tibial plateau of WT and SOST-KO OA mice (n = 5 animals at each time point). Data are mean ± SEM. #P < 0.05.