Table 1 Gene primer sequences used in RNA-Seq validation
From: Transcriptome analysis of ageing in uninjured human Achilles tendon
Name | Gene type | Ensemble gene ID/transcript ID | Primer sequence |
|---|---|---|---|
RPS16 | Protein-coding | ENSG00000105193 | F: GAAATCCTACCGATAAGCCCA |
R: TTTCTTGAAACTTTAAAATCCCTCAA | |||
MMP3 | Protein-coding | ENSG00000149968 | F: ATTCCATGGAGCCAGGCTTTC |
R: CATTTGGGTCAAACTCCAACTGTG | |||
COL1A1 a | Protein-coding | ENSG00000108821 | F: GGAGGAGAGTCAGGAAGG |
R: GCAACACAGTTACACAAGG | |||
ACAN b | Protein-coding | ENSG00000108821 | F: TCGAGGACAGCGAGGCC |
R: TCGAGGGTGTAGCGTGTAGAGA | |||
POU3F4 | Protein-coding | ENSG00000196767 | F: GTTCGCTCGCTCTCTCGTA |
R: GAAGGGAAGGGAAGGGGAAA | |||
MYF5 | Protein-coding | ENSG00000111049 | F: TGAACTAATTTTCTGGTCTATATGAC |
R: TGTACATGTTGTCTTGGTTTGGG | |||
IGF1 | Protein-coding | ENSG00000017427 | F: CACTATGGACAGATGTAAAAGAAACTA |
R: ACACACTGGGGACAAGAAATAAA | |||
MMP16 | Protein-coding | ENSG00000156103 | F: ACCCGTGTAACCCTTTGAGA |
R: AACCTGAACTTCTTGAACTTGTG | |||
TGFB3 | Protein-coding | ENSG00000119699 | F: GTAAAGAAAGTGTGGGTTTGGTTA |
R: AACATCTCAACTTACCATCCCTTT | |||
EGF | Protein-coding | ENSG00000138798 | F: ACAGGAGGCTTCGGAGTT |
R: AATCAGGCAATTTACTTACAATCTT | |||
COL3A1-001 | Protein-coding | ENST00000304636 | F: CAGGTCCCAGCGGTTCT |
R: CCTTTTGGTCCAGACACTCC | |||
COL3A1-201 | Protein-coding | ENST00000317840 | F: GGTAGCCCTGGTGAGAGAG |
R: TGCCAGGAGGTCCAAAGAG | |||
FGF10-001 | Protein-coding | ENST00000264664 | F: TGCCGTCAAAGCCATTAACA |
R: CATTTTCCTCTATCCTCTCCTTCA | |||
CRTAC1-001 | Protein-coding | ENST00000370597 | F:ATCTTCTTCAACAACATTGCCTAC |
R: GGGTCTCCGTGCTCTCTAC | |||
IGF1-001 | Protein-coding | ENST00000337514 | F: CAGCAGTCTTCCAACCCAAT |
R: AAGAGATGCGAGGAGGACAT | |||
XIST | Lnc | ENSG00000229807 | F: TCCCAGAGAATGCCTAATACTTT |
R: GTAGAAGAGATACGGAGTAGGAAT | |||
LINC00957 | Lnc | ENSG00000235314 | F: GAGAGTAAGCAGACCTGGGT |
R: ACCTTGTCCGAGTTCCATCT | |||
RP11.308Â N19.1 | Lnc | ENSG00000234323 | F: GCCTCTTTCATCACTGCCGA |
R: TAGCAGCAGTTGGGGTGTTT |