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Table 10 Real-time polymerase chain reaction analysis of 14 selected genes reveals good correlation with RNA-Seq results

From: Transcriptome analysis of ageing in uninjured human Achilles tendon

Gene name

RNA-Seq results

RT-PCR results

Age

P value

Differential expression

Significant log 2 fold change

q value

Young

Old

 

EGF

Higher in old

6.50

2.06 × 10−8

0.003 ± 0.002

0.01 ± 0.007

0.04

POU3F4

8.20

7.79 × 10−16

0.021 ± 0.015

0.245 ± 0.147

0.02

MYF5

6.30

0.003

0.061 ± 0.057

0.25 ± 0.07

0.01

TGFB3

Lower in old

−1.60

0.035

10.717 ± 6.022

0.897 ± 0.34

0.05

MMP16

−2.30

0.022

0.107 ± 0.087

0.014 ± 0.02

0.07

COL3A1

−3.70

0.008

13.696 ± 17.535

1.05 ± 0.594

0.27

MYH1

−4.60

0.003

0.288 ± 0.155

0.14 ± 0.101

0.23

IGF1

−2.10

0.004

2.094 ± 1.234

0.483 ± 0.278

0.04

COL1A1

−3.30

0.009

47.968 ± 32.676

8.515 ± 9.154

0.42

MMP3

No change

Not significant

1.000

51.181 ± 362.11

99.323 ± 66.10

0.15

ACAN

1.000

1.439 ± 1.43

0.848 ± 0.99

0.66

XIST

Higher in old

6.90

0.000

0.827 ± 0.712

7.41 ± 3.465

0.03

LINC00957

Lower in old

−1.70

0.036

1.044 ± 0.584

0.363 ± 0.174

0.08

RP11.308 N19.1

 

−2.430

0.040

0.02 ± 0.065

0.005 ± 0.001

0.03

  1. Values for real-time polymerase chain reaction (RT-PCR) are the mean ± standard deviation of relative expression levels normalised to expression of RSP16 (to two decimal places). Statistical significance was tested by using Student’s t test. RT-PCR results are expressed as 2^-DCT. ACAN, aggrecan; COL1A1, collagen type 1 alpha 1; COL3A1, collahen type 3 alpha 1; EGF, epidermal growth factor; IGF1, insulin growth factor 1; LINC00957 long intergenic non-protein coding RNA 957; MMP3, matrix metalloproteinase; MMP16, matrix metalloproteinase 16; MYF5, myogenic factor 5; MYH1, myosin heavy chain 1; POU3F4, POU class 3 homeobox 4; RP11.308 N19.1, Inc-ZNF462-2; TGFB3, transforming growth factor β.
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Arthritis Research & Therapy

ISSN: 1478-6362

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