MicroRNA-33a regulates cholesterol synthesis and cholesterol efflux-related genes in osteoarthritic chondrocytes

Table 1 Oligonucleotide primers used in cDNA synthesis for the detection of miR-33a and U6 (stem-loop primers) and for real-time quantitative PCR assay ^a

	RT-qPCR stem-loop primer
miR-33a	5′-TGGATATCCACACCAGGGTCCGAGGTATTCGGTGTGGATATCCATGCAATG
U6	5′-CACGGAAGCCCTCACACCGTGTCGTTC
Gene	Forward primer sequence	Reverse primer sequence
miR-33a	CGCGCGTGCATTGTAGTTG	CACCAGGGTCCGAGGT
U6	GCTTCGGCAGCACATATACTAAAAT	CTCACACCGTGTCGTTCCA
SREBP2	AAGTCTGGCGTTCTGAGGAA	AGGTCCACCTCATTGTCCAC
ABCA1	GGAGGCAATGGCACTGAGGAA	CCTGCCTTGTGGCTGGAGTGT
ApoA1	ATGAAAGCTGCGGTGCTGACC	CACCTTCTGGCGGTAGAGCTCC
LXRα	CCGCCTGAAGAAACTGAA	CGAAGCCGGTCAGAAAAG
LXRβ	CGCTACAACCACGAGACAGA	GTGGAAGTCGTCCTTGCTGT
MMP-13	TGGCATTGCTGACATCATGA	GCCAGAGGGCCCATCAA
Smad7	TCCAGATACCCGATGGATTTTC	GATTTTGCTCCGCACCTTCT
GAPDH	GAGTCAACGGATTTGGTCGT	GACAAGCTTCCCGTTCTCAG

^aABCA1, ATP-binding cassette transporter A1; ApoA1, Apolipoprotein A1; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; LXR, Liver X receptor; miR-33a, MicroRNA-33a mature; MMP-13, Matrix metalloproteinase-13; SREBP2, Sterol regulatory element-binding protein 2.

ISSN: 1478-6362