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Table 2 Composition of the staining panels for human B-cell phenotyping

From: Polychromatic flow cytometry in evaluating rheumatic disease patients

  Memory panel Transitional panel Plasma cell panel
Fluorochrome Specificity Clone Specificity Clone Specificity Clone
FITC IgD IA6-2 MTG Free dye Ki-67 B56
PE CXCR3 1C6 IgD   CXCR3 1C6
PE-Alexa610 CD24 SN3 CD24   CD24  
PE-Cy5 CD21 B-ly4 IgM G20-127 CXCR4 12G5
PerCP-Cy5.5 CD38 HIT2 CD38   CD3  
PE-Cy7 CD45/B220 RA3/6B2 CD23 EBVCS2 CD38  
Pacific Blue CD3 SP34-2 CD3   VH4-34  
Pacific Orange Live/Dead Aqua Live/Dead   Live/Dead  
Qdot605 CD27 CLB-27/1 CD27   CD27  
APC CD95 DX2 CD10 HI10a CD138 B-B4
Alexa680 VH4-34 9G4 VH4-34   IgD  
APC-Cy7 CD19 SJ25C1 CD19   CD19  
  1. The seven anchor markers and VH4-34 encoded heavy chain are in bold font. Information on the detecting antibody clones for these eight molecules is omitted from the transitional and plasma cell panels, as they are the same as those indicated under the memory panel regardless of the fluorochrome conjugates. For detection in the Red B channel, antibodies used are biotinylated and detected by streptavidin-Alexa680. Note that in the plasma cell panel, both Ki-67 and 9G4 are intracellular staining. APC, allophycocyanin; Aqua, LIVE/DEAD fixable aqua dead cell stain; Cy, cyanine; FITC, fluorescein isothiocyanate; PE, R-phycoerythrin; PerCP, peridinin-chlorophyll protein; Qdot, quantum dot.