Figure 2From: Effect of coculturing canine notochordal, nucleus pulposus and mesenchymal stromal cells for intervertebral disc regeneration Extracellular matrix deposition. Histopathological slides of typical cell morphologies on day 28 of notochordal cells (NCs), mesenchymal stromal cells (MSCs), nucleus pulposus cells (NPCs), articular chondrocytes (ACs), MSC + NC, NPC + NC, NPC + MSC, MSC + AC, and NPC + AC. Prior to staining, alginate was removed with sodium citrate. Cell nuclei are stained blue (hematoxylin), proteoglycans are red (Safranin O) and collagen is green (Fast Green) (scale bar = 50 μm).Back to article page