Figure 2
From: CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women
Effect of rs1790834 genotypes on expression of CYB5A and steroidogenic genes. Real-time quantitative PCR analysis of mRNA expression in 22 RA synovial fibroblast lines (rs1790834 genotype GG, n = 15; AA/AG genotypes, n = 7). (A) Synovial fibroblasts express all genes, which are necessary for synthesis of biologically active androgens. Mean ± SD of expression levels normalized to HPRT1 of combined data for all genotypes. (B) rs1790834 genotype-dependent expression levels, which are normalized to the median value of the combined expression data for each gene. In the box-and-whisker plots the genotypes with the rare allele A were grouped together (AA, n = 1; AG, n = 6). The boxes represent the 25th to 75th percentiles, and horizontal lines within the box represent median values. The whiskers show the 10th and 90th percentiles, respectively, and dots represent individual values exceeding these limits. ***Significant rs1790834 genotype-dependent expression, P <0.005, Mann-Whitney rank-sum test. CYB5A, cytochrome b5, type A; CYP17A1, steroid 17α-hydroxylase/17,20-lyase (cytochrome P450 17A1); CYP19A1, aromatase (cytochrome P450 19A1); CYP7B1, 25-hydroxycholesterol 7α-hydroxylase (cytochrome P450 7B1); HPRT1, hypoxanthine(−guanine) phosphoribosyltransferase 1; HSD3B2/7, 3β-hydroxysteroid dehydrogenase isoforms 2/7; POR, NADPH-cytochrome P450 reductase.