Table 1 Growth factor and cytokine profiling in systemic sclerosis and control blister fluid samples
Biologic function | Healthy control samples | SSc samples | P value | |
|---|---|---|---|---|
Innate immunity | IFNα2 | 5.34 (4.4 to 7.61) | 6.35 (4.81 to 7.52) | 0.35 |
IL-1α | 74.77 (22.48 to 132.28) | 40.42 (15.27 to 113.19) | 0.44 | |
IL-1B | 0.37 (0.37 to 1.62) | 0.62 (0.37 to 1.62) | 0.57 | |
IL-1RA | 802.5 (469.25 to 1,796.5) | 695 (499.5 to 1,075.5) | 0.72 | |
IL-6 | 5.51 (2.44 to 26.83) | 38.78 (19.23 to 88.81) | 0.01 | |
IL-12p40 | 5.16 (4.39 to 6.11) | 4.58 (4.39 to 7.6) | 0.86 | |
IL-12p70 | 2.03 (1.74 to 2.21) | 1.99 (1.72 to 2.34) | 0.87 | |
IL-15 | 4.38 (3.2 to 4.95) | 6.27 (4.21 to 9.34) | 0.03 | |
IP-10 | 988.5 (510.5 to 1,172.25) | 1054 (547.25 to 1,929) | 0.44 | |
TNFα | 25.95 (12.15 to 65.36) | 22.19 (14.46 to 44.1) | 0.92 | |
Adaptive immunity | IFNγ | 1.23 (1.12 to 2.76) | 1.58 (1.33 to 2.76) | 0.19 |
IL-2 | 1.26 (0.73 to 1.26) | 1.26 (0.64 to 1.31) | 0.57 | |
IL-3 | 1.62 (1.14 to 1.62) | 1.62 (1.1 to 1.62) | 0.78 | |
IL-4 | 2.6 (1.72 to 5.24) | 3.955 (2.18 to 5.24) | 0.54 | |
IL-5 | 1.28 (0.84 to 1.72) | 0.96 (0.84 to 1.72) | 0.83 | |
IL-7 | 4.16 (2.99 to 5.59) | 4.89 (3.52 to 7.94) | 0.13 | |
IL-9 | 0.77 (0.77 to 1.94) | 0.77 (0.77 to 1.94) | 0.86 | |
IL-10 | 22.02 (16.77 to 40.28) | 33.05 (12.91 to 44.83) | 0.83 | |
IL-13 | 1.95 (1.67 to 2.8) | 1.98 (1.66 to 2.8) | 0.81 | |
IL-17a | 1.23 (1.23 to 1.34) | 1.23 (1.23 to 1.34) | 0.78 | |
sCD40L | 284.5 (207.5 to 466.5) | 285 (204.75 to 483) | 0.83 | |
TNFβ | 1.04 (1.04 to 3.95) | 1.04 (1.04 to 3.95) | 0.97 | |
Chemokines | Eotaxin | 37.95 (21.05 to 48.53) | 31.46 (26.64 to 48.93) | 0.92 |
Fractalkine | 37.96 (30.03 to 52.69) | 39.61 (22.83 to 48.54) | 0.62 | |
GRO | 69.91 (47.16 to 252) | 129 (78.92 to 246.25) | 0.31 | |
IL-8 | 61.85 (28.05 to 234.75) | 51.69 (37.24 to 80.03) | 0.66 | |
MCP-1 | 762.5 (544 to 1,661.25) | 792 (668.75 to 1,340.5) | 0.92 | |
MCP-3 | 7.18 (5.7 to 9.81) | 9.81 (7.5 to 10.66) | 0.04 | |
MDC | 1004.5 (715 to 1,126.75) | 695 (554.5 to 867.25) | 0.10 | |
MIP-1α | 21.21 (3.51 to 47.18) | 10.5 (3.51 to 16.46) | 0.31 | |
MIP-1β | 43.44 (8.13 to 111.17) | 24.42 (12.89 to 45.65) | 0.46 | |
RANTES | 47.21 (19.68 to 178.01) | 55.76 (33.4 to 101.35) | 0.71 | |
Growth factors | EGF | 2.72 (2.72 to 10.12) | 2.72 (2.72 to 7.25) | 0.58 |
Flt-3 L | 70.25 (4.16 to 82.49) | 51.82 (23.89 to 77.59) | 0.45 | |
GCSF | 3.26 (3.24 to 16.04) | 3.24 (3.23 to 12.33) | 0.69 | |
GMCSF | 2.79 (1.88 to 9.33) | 2.22 (1.74 to 7.24) | 0.63 | |
PDGF-AA | 6.94 (5.87 to 7.78) | 10.38 (7.4 to 17.71) | 0.03 | |
PDFG-BB | 1.79 (0.41 to 1.79) | 1.79 (1.79 to 3.2) | 0.23 | |
TGF-α | 7.09 (6.64 to 8.21) | 7.0 (5.62 to 9.1) | 0.62 | |
FGF-2 | 13.4 (11.67 to 16.8) | 16.92 (13.4 to 20.24) | 0.09 | |
VEGF | 11.3 (6.92 to 16.92) | 11.67 (10.28 to 22.29) | 0.47 |