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Figure 5 | Arthritis Research & Therapy

Figure 5

From: Altered balance of interleukin-13/interferon-gamma contributes to lacrimal gland destruction and secretory dysfunction in CD25 knockout model of Sjögren’s syndrome

Figure 5

Comparison of apoptosis among different double knockouts. (A) Representative merged images of laser scanning immunofluorescent confocal micrographs of LG immunostained for IFN-γ receptor (IFN-γR, green) with propidium idodide counterstaining (red) in IL-17KO, CD25KO, CD25/IL-17 DKO, CD25/IL-17/IFN-γ TKO, IL-17/IFN-γ DKO and C57BL/6. Scale bar = 50 μm. (B-C) Relative fold of expression of Fas-L and TRAIL (B) and caspase-3, -8 and -9 (C) in LG lysates of IL-17KO, CD25/IL-17 DKO and CD25KO mice aged 4 to 8 weeks (W). Data are presented as mean ± SEM of eight individual samples/strains/ages. (D) Caspase-3, -8 and -9 activity assay results from LG lysates of IL-17KO, CD25/IL-17 DKO, CD25KO and CD25/IFN-γ DKO mice aged 4 to 8 W. Data are presented as mean ± SEM of five to eight individual samples/strains/ages. (E) Representative merged images of laser scanning fluorescent confocal micrographs of TUNEL-stained LG (green) with propidium iodide counterstaining (red) in IL-17KO, CD25KO, CD25/IL-17 DKO, CD25/IL-17/IFN-γ TKO, IL-17/IFN-γ DKO, C57BL/6 and CD25/IFN-γ DKO mice used to generate the bar graphs in (F) Scale bar = 50 μm. F. TUNEL+ cells (mean ± standard deviation (SD)) among the different groups. * P <0.05; ** P <0.01, *** P <0.001; **** P <0.0001 within strain comparison vs. 4 W. ^ P <0.05; ^^ P <0.01, ^^^ P <0.001; ^^^^ P <0.0001 interstrain comparison. CD25/IFN-γ DKO, CD25−/−IFN-γ−/−; CD25/IL-17/IFN-γ TKO, CD25−/−IL-17−/−IFN-γ−/−; CD25KO, CD25 knockout; DKO, double knockout; Fas-L, Fas-ligand; IFN-γ, interferon-gamma; IL-17/IFN-γ DKO, CD25+/−IL-17−/−IFN-γ−/−; IL, interleukin; IL-17KO, IL-17 knockout; LG, lacrimal gland; SEM, standard error of the mean; TKO, triple knockout; TNF, tumor necrosis factor; TRAIL, TNF-related apoptosis-inducing ligand; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; W, weeks.

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