Cytotoxicity assay of neutrophils with different stimulations and dose–response curves for involved blocking antibodies and inhibitors. LDH release of neutrophils incubated with anti-TLR2 at 5 μg/ml, anti-TLR4 at 5 μg/ml, RAGE-Fc at 5 μM, MyD88 inhibitor at 20 μM or NF-κB inhibitor were measured. Involved blocking antibodies and inhibitors showed no significantly cytotoxicity (A) activating effect (B-F) to neutrophils. B-F also showed dose–response curves for involved blocking antibodies and inhibitors. Bars represent mean ± standard deviation (SD) of repeated measurements of three independent experiments. LDH, lactate dehydrogenase; MyD88, myeloid differentiation factor 88; NF-κB, nuclear factor kappa B; RAGE, receptor for advanced glycation end products; TLR, Toll-like receptor.