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Figure 6 | Arthritis Research & Therapy

Figure 6

From: High mobility group box 1 contributes to anti-neutrophil cytoplasmic antibody-induced neutrophils activation through receptor for advanced glycation end products (RAGE) and Toll-like receptor 4

Figure 6

Western blot analysis for MyD88 and phospho-NF-κ B. Neutrophils were stimulated with HMGB1 10 ng/ml or buffer for 30 min, certain groups followed by stimulation with ANCA-positive IgG. Samples were harvested and MyD88 and phospho-NF-κB were determined by immunoblotting. A representative example is shown. GAPDH is shown as loading control (A and D). The corresponding densitometric analysis was shown in B, C, E and F (n = 3). * P <0.05. Bars represent mean ± standard deviation (SD) of repeated measurements of 3 independent experiments. ANCA, antineutrophil cytoplasmic antibody; HMGB1, high mobility group box-1; IgG, immunoglobulin G; MyD88, myeloid differentiation factor 88; NF-κB, nuclear factor kappa B.

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