Western blot analysis for MyD88 and phospho-NF-κ
B. Neutrophils were stimulated with HMGB1 10 ng/ml or buffer for 30 min, certain groups followed by stimulation with ANCA-positive IgG. Samples were harvested and MyD88 and phospho-NF-κB were determined by immunoblotting. A representative example is shown. GAPDH is shown as loading control (A and
D). The corresponding densitometric analysis was shown in B, C, E and F (n = 3). *
P <0.05. Bars represent mean ± standard deviation (SD) of repeated measurements of 3 independent experiments. ANCA, antineutrophil cytoplasmic antibody; HMGB1, high mobility group box-1; IgG, immunoglobulin G; MyD88, myeloid differentiation factor 88; NF-κB, nuclear factor kappa B.