Figure 6

Modulation of T cell activation by ROS. (A) One representative flow cytometry analysis of CD69 expression is shown. PMA activated normal T cells (upper panels) or SSc T cells (lower panels) were treated with DPI (20 μM, 1 hour). Plots represent live cells gated for CD4+ cells. (B) Percentage of CD69+ cells in three healthy controls (upper panel) and three SSc patients (lower panel) are presented. Cells were treated as in A. Data are means ± standard deviation (SD). ^* P <0.05 compared to untreated T cells. (C) One representative flow cytometry analysis of CD69 expression is shown. Normal T cells (upper panels) or SSc T cells (lower panels) were activated with CD3/CD28 magnetic beads and treated with DPI (20 μM, 1 hour). Plots represent live cells gated for CD4+ cells. (D) Percentage of CD69+ cells in three healthy controls (upper panel) and three SSc patients (lower panel) are presented. Cells were treated as in C. Data are means ± SD. ^* P <0.05 compared to untreated T cells. DPI, diphenylene iodonium; PMA, phorbol myristate acetate; ROS, reactive oxygen species; SSc, systemic sclerosis.