Neutralization of IL-10 exacerbates collagen-induced arthritis in RORγt Tg mice. (A) C57BL/6 mice (n = 5) and RORγt Tg mice (n =4) were immunized intradermally with chicken type II collagen (CII) emulsified with complete Freund’s adjuvant (CFA) on days 0 and 21. Anti-IL-10 monoclonal antibody (mAb) or isotype control antibody (ctrl Ab) were administrated intraperitoneally every 2 days from day 22 to 30 post first CII immunization. Severity of arthritis was shown. Representative data are mean ± standard error of the mean (SEM) values. *P <0.05 versus C57BL/6 mice with the administration of isotype ctrl Ab, #
P <0.05 versus RORγt Tg mice with the administration of isotype ctrl Ab, Student’s t-test. (B) At day 10 post first CII immunization, CD4+ cells were isolated by magnetic-activated cell sorting (MACS) from draining lymph-node cells of C57BL/6 mice (open bars) and RORγt Tg mice (closed bars), and added to the upper well of a Transwell system. The migration assay was performed in the presence of CCL20 and anti-IL-10 mAb or isotype ctrl Ab added to the bottom well. Data are number of respective cells that migrated to the bottom well counted by FACS. Representative data of two independent experiments with similar results and are mean ± SEM value.