Regulation of large intergenic noncoding RNAs in peripheral blood mononuclear cells by Pam3CSK4. (A) Expression of the large intergenic noncoding RNA linc0949 was lower in untreated and stimulated peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE) than in those of healthy controls. linc0949 was suppressed by the Toll-like receptor 2 (TLR2) ligand Pam3CK4 (20 ng/ml) in healthy controls, and it was not significantly different after treatment in patients with SLE. (B)
linc0597 was upregulated by TLR2 agonist Pam3CK4 (20 ng/ml) in PBMCs of healthy controls, whereas it did not respond to the stimuli in patients with SLE. linc0597 expression was significantly decreased in PBMCs of patients with SLE compared with healthy controls both before stimulation and after treatment. The data shown are from five healthy donors and five patients with SLE after incubation with the indicated stimuli for 4 hours. Real-time quantitative PCR of the indicated RNAs is normalized to RPL13A levels (mean ± standard deviation (SD)). Data are mean ± SD. P-values were determined by performing unpaired t-tests. NS, Not significant. *P < 0.05; **P < 0.01.