Figure 2

Histogram representation of the levels of phosphorylated p38, NF-kB and Erk1/2. (A-C) Levels of phosphorylated p38 (A), NF-kB (B) and Erk1/2 (C) responding to in vitro stimulation with recombinant tumor necrosis factor (TNF)-α in healthy donors (top panel), and rheumatoid arthritis patients prior to (middle panel) and 1 month following TNF-α inhibitor treatment (bottom panel). Lighter colored histograms indicate higher median signal intensity. Within each box, upper histograms represent the stimulated sample; lower histograms represent the unstimulated control sample. All plots were generated in Cytobank [50]. NF, nuclear factor; NK, natural killer.