Table 2 Murine primer pairs used for quantitative real-time PCR
Gene | Primer | Product | Sequence (5′-3′) |
|---|---|---|---|
Actb | mβ-Act_885for | 348 bp | TGGAATCCTGTGGCATCCATGAAAC |
mβ-Act_1233rev | TAAAACGCAGCTCAGTAACAGTCCG | ||
Acan | mAggrecan_1922for | 206 bp | CAGTTCACCTTCCAGGAAG |
mAggrecan_2128rev | GTAGAGGTAGACCGTTCTCACG | ||
Col2a1 | mColl2_2657for | 261 bp | CTACTGGAGTGACTGGTCCTAAGG |
mColl2_2918rev | GGACCATCATCTCCAGGTTCTCC | ||
Col10a1 | mCol10_38for | 287 bp | CTGCCCCACGCATCTCCCAG |
mCol10_325rev | GCTTGCCTGGCGGTCCTGAG | ||
Mmp1a/Mmp1b | mMMP1_17for | 175 bp | CTGTTGCTTCTCTGGGCTGC |
mMMP1_192rev | CTGCATTTGCCTCAGCTTTTC | ||
Mmp3 | mMMP3_362for | 104 bp | GTTCCTGATGTTGGTGGCTTCAG |
mMMP3_466rev | CTGTCTTGGCAAATCCGGTGTA | ||
Mmp13 | mMMP13_899for | 80 bp | CCCAGCCCTATCCCTTGATGCCA |
mMMP13_979rev | TGCAGGCGCCAGAAGAATCTGT | ||
Tfap2a | mAP-2α_1170for | 69 bp | GCGGCCCAATCCTATCCT |
mAP-2α_1238rev | CCATGGGAGATGAGGTTGAAG | ||
Tfap2b | mAP-2β_1355for | 67 bp | AAAGCTGTCTCACGCACTTCAGT |
mAP-2β_1421rev | AGCGCAGCGCAAATGG | ||
Tfap2c | mAP-2γ_1115for | 537 bp | ACCTAGCACGGGACTTCGCCT |
mAP-2γ_1651rev | GGGCGGGCGGGTTGTAACTG | ||
Tfap2e | mAP-2ε_388for | 154 bp | GCCGACCCTGGGGAGCTACAC |
mAP-2ε_542rev | CACCTCCGGCGCCGCTTAAA | ||
Timp1 | mTIMP1_147for | 270 bp | AGACACACCAGAGCAGATACC |
mTIMP1_417rev | CCGGATATCTGCGGCATTTC | ||
Timp2 | mTIMP2_442for | 280 bp | GCAGACGTAGTGATCAGAGCC |
mTIMP2_722rev | TCCCAGGGCACAATGAAGTC | ||
Timp3 | mTIMP3_255for | 315 bp | GACCCTTGGCCACTTAGTCC |
mTIMP3_570rev | CGGATCACGATGTCGGAGTTG |