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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Toll-like receptor 2 (TLR2) induces migration and invasive mechanisms in rheumatoid arthritis

Fig. 5

a Representative photomicrographs of RASFC inhibition of migration by anti-β1-integrin (10 μg/ml) are seen in (i). Bar graphs showing RASFC (ii) and HMVEC (iii) repopulating the wound in response to Pam3CSK4 (1 μg/ml), an effect that is inhibited by anti-β1-integrin (10 μg/ml) (n = 4). b Representative photomicrographs showing anti-β1-integrin (10 μg/ml) inhibits Pam3CSK4 (1 μg/ml)-induced RASFC invasion (i). Bar graph quantifying RASFC invasion (ii) (n = 4). c Western blot for Rac1, total Rac1 and β-actin control demonstrating that Pam3CSK4 (1 μg/ml)-induced Rac1 activation in RASFC is inhibited by the presence of anti-β1-integrin (10 μg/ml), with no effect observed for anti-IgG control monoclonal antibody (10 μg/ml). Total Rac1 was unchanged (n = 1) Data is represented as mean ± SEM, * p <0.05, ** p <0.01, significantly different to control. HMVEC human microvascular endothelial cells, IgG immunoglobulin G, RASFC rheumatoid arthritis synovial fibroblast cells

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