Skip to main content
Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Anti-citrullinated protein antibodies contribute to platelet activation in rheumatoid arthritis

Fig. 1

Plasma-derived factors enhance platelet activity. Platelets were isolated from buffy coat and incubated in the presence of plasma obtained from patients with rheumatoid arthritis (RA) or age- and sex-matched healthy controls (cohort 1). Plasma from patients with RA induced higher platelet activation than did plasma from healthy donors, as indicated by (a) increased percentage and (b) mean fluorescence intensity (MFI) of P-selectin (CD62P) expression, (d) platelet aggregation and (f) soluble CD40 ligand (sCD40L). c Histogram overlay showing representative examples of CD62P expression. e Representative pictures of aggregate formation and CD62P expression of non-aggregated and aggregated platelets. f Values of sCD40L were corrected for amounts present in plasma. Levels of (g) P-selectin and (h) sCD40L production correlated with high levels of anti-citrullinated protein antibodies (ACPA; > 1000 AU/ml). Each symbol represents a plasma sample. FSC forward scatter, SSC side scatter

Back to article page