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Table 1 Primer sequences for PCR, real-time PCR, methylation-specific PCR (MSP), quantitative MSP (Qmsp) and bisulfite (Bis.) sequencing analysis

From: DNA methylation regulates sclerostin (SOST) expression in osteoarthritic chondrocytes by bone morphogenetic protein 2 (BMP-2) induced changes in Smads binding affinity to the CpG region of SOST promoter

Name

Sequence (5′-3)

Experiment

M-SOST-forward

GAATAGGTCGGGTTTAGTTTC

MSP, qMSP

M-SOST-reverse

ACCTCCCACGTACTAACGA

MSP, qMSP

U-SOST-forward

GGAATAGGTTGGGTTTAGTTTT

MSP, qMSP

U-SOST-reverse

CACCTCCCACATACTAACAA

MSP, qMSP

SOST-forward

CCGGAGCTGGAGAACAACAAG

RT-PCR

SOST-reverse

GGTGTGCTCCGGCCAGTGC

RT-PCR

Promoter SOST-forward

GGGACCAATGGGATTTCTTT

PCR

Promoter SOST-reverse

TGAGCTCCGGCTTTTAATTG

PCR

BSP SOST-forward

TTATTTGTTGGTGGGGTGATAA

Bis. sequencing

BSP SOST-reverse

ACAAAACCCAAACCTACTCTCC

Bis. sequencing