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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Autoantigen microarrays reveal autoantibodies associated with proliferative nephritis and active disease in pediatric systemic lupus erythematosus

Fig. 1

Autoantigen microarrays identify autoantibodies associated with pediatric SLE. Serum samples from 45 new-onset pSLE patients and 17 age-matched healthy controls were used to probe autoantigen microarrays featuring 140 purified or recombinant autoantigens. Cy5-conjugated anti-human IgG antibodies were used as a secondary reagent, a fluorescent microarray scanner was used to image each microarray, and the feature MFIs were used to quantify binding. Significance analysis of microarrays (SAM) was used to identify significant differences in IgG reactivity to the autoantigens between pSLE patients and controls (q-value <0.001, fold change >2). A hierarchically clustered heatmap (complete linkage, unsupervised) of the significant autoantigens is shown, with pSLE patients indicated on the top bar in purple and healthy controls in green. Patient clusters are colored red, blue green and magenta in the phylogenetic tree. IgG immunoglobulin G, MFI median fluorescence intensity, pSLE pediatric systemic lupus erythematosus, SLE systemic lupus erythematosus

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