Fig. 3

Autoantigen microarrays identify multiple autoantibodies associated with proliferative nephritis in pSLE. Serum samples from 41 new-onset pSLE patients were used to probe autoantigen microarrays featuring 140 purified or recombinant autoantigens. Anti-human IgG antibodies conjugated with Cy5 were used as a secondary reagent, a fluorescent microarray scanner was used to image each microarray, and the feature MFIs were used to quantify binding. Significance analysis of microarrays (SAM) was used to identify significant differences in IgG reactivity to the autoantigens between patients with biopsy-proven class III/IV nephritis and patients without significant evidence of nephritis (q-value <0.06, fold change >2). A hierarchically clustered heatmap of the significant antigens is shown. Patients with biopsy-proven proliferative nephritis are indicated on the top bar in red, while patients without nephritis are shown in blue. IgG immunoglobulin G, MFI median fluorescence intensity, pSLE pediatric systemic lupus erythematosus, PN proliferative nephritis